Thanks a lot.
I am doing a metagenomics study.
I think I can start with my trimming, because most of the bases have a quality better than q10, so I only have to erase short ones.
I will try the freeware you told me.
Cheers
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Originally posted by miguelangel View PostHello there!
I'm starting with my brand new data from Roche's 454 pyrosequencing and I have some questions at this point.
First of all, I am not very confident with my .qual files. I have sequences with bases with no less than q10. Is it allright to start my personal trimming? The people who have run the analysis has also done one before, so I understand that low quality bases have been deleted.
Second question: which freeware can I use to work with my .sff files?
Thanks a lot, I hope this questions are not too stupid and that you won't mind to help me.
Cheers, Miguel Ángel
The freeware software to make an assembly can be Celera, I've tested this and I have good results (sometimes better than in newbler), MIRA is another software but I didnt use it.
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hello and first questions!
Hello there!
I'm starting with my brand new data from Roche's 454 pyrosequencing and I have some questions at this point.
First of all, I am not very confident with my .qual files. I have sequences with bases with no less than q10. Is it allright to start my personal trimming? The people who have run the analysis has also done one before, so I understand that low quality bases have been deleted.
Second question: which freeware can I use to work with my .sff files?
Thanks a lot, I hope this questions are not too stupid and that you won't mind to help me.
Cheers, Miguel Ángel
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