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  • #16
    Originally posted by exon View Post
    Anyone also work with high cpb with improved enrichment percentage?

    We also suffered from low enrichment problem, mostly below 2%. The template that we are using is amplicon with fusion primer (with adaptor and barcode). We have checked the template with bioanalyzer and KAPA qPCR first. Then we used 1.7 cpb in the LV Lib-L emPCR. The enrichment is lower than 2% that we suspected the cpb not optimize, emPCR failed or the problem of the template? Then we went for SV using various cpb from 0.25 to 17, again the enrichment was <2% while the positive control (from previous work fine library) is OK but cpb increased from 1.7 to 14.6. We have already repeated SV in three times and used up the reagents.
    Unfortunately I cannot help you as I still do not know what causes this. I can only tell you that for me personally this is a trial and error situation still (since months now). Good luck.

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