Originally posted by exon
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preincubation of enrichment beads with enrichment primer
Originally posted by HMorrison View PostSo, did it help? We are still doing it. Current work-around is to underload the plate and simply expect to have to do two runs to get the number of reads that we routinely got up until Dec 2011. Been nearly a full year of this, now.
Yes, preincubation of the enrichment beads with the enrichment primer as you suggested worked in our LV lib-L. The problem now is that the enrichments is now suddenly 40%, while from the SV (done without preincubation step) with the same cpb (1,5) gave 5% enrichment.
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