Tweet
Last week, we ran our first Ti plate with the signal processing during the run (vs. doing only image collection then signal afterwards). The images were all collected properly and looked likel signal processing was fine until ~step 130 where it caused the SW to crash and kick out to the adminrig login screen with the red flashing light. I logged back in and took the images and did runAnalysisPipe and everything was fine. Any similar experience for others?
-
-
What version of the Newbler pipeline do you have? -
we're running 2.0.01.12. We've recently noticed too that the signal processing is not completing (i.e. no Wells tab/information) so we have to do the signal processing on the datarig (same SW but no problems).Comment
-
I was hoping someone else would reply with a more authoritative answer. However, I have a vague recollection from a local next gen meeting of someone mentioning this was a known issue with a recent Newbler update (hence my question about which version you were using).
I'd suggest you check with your Roche contact to see if this is the case.Comment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment