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  • unidentified base (N) in 454 reads

    Hello All,

    Do we need to trim or discard 454 reads on the basis of N present in the reads. If we should discard reads on this basis, what should be the ideal cut off. How many N s per reads is okay to accept or what percentage of N should be allowed per read.

    Any suggestion or reference to related paper is appreciated.

    Thanking you.

    Bagdevi.

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