Has anyone performed assemblies with GSAssembler (ver. 2.0.01) using Sanger data (fasta) in combination with 454 data? A friend has done this for me with miraEST and I was going to try it with Newbler. Just wondering what opinions were as to the quality of similar assemblies.
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Any particular reason for using Newbler gsAssembler v2.0.01 when v2.3 is out? As I recall, the main new feature is better support for EST assemblies. -
Good point! I got this ver. from our core facility, so maybe they gave me the wrong install disk since this summer the Roche rep. said it was the latest "hot off the presses" version. I should be able to get the latest ver. So, have you done this type of assembly with the new version?Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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