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  • Performing Hybrid Assemblies With Newbler

    Has anyone performed assemblies with GSAssembler (ver. 2.0.01) using Sanger data (fasta) in combination with 454 data? A friend has done this for me with miraEST and I was going to try it with Newbler. Just wondering what opinions were as to the quality of similar assemblies.

  • #2
    Any particular reason for using Newbler gsAssembler v2.0.01 when v2.3 is out? As I recall, the main new feature is better support for EST assemblies.

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    • #3
      Good point! I got this ver. from our core facility, so maybe they gave me the wrong install disk since this summer the Roche rep. said it was the latest "hot off the presses" version. I should be able to get the latest ver. So, have you done this type of assembly with the new version?

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      • #4
        Originally posted by WaltL View Post
        Good point! I got this ver. from our core facility, so maybe they gave me the wrong install disk since this summer the Roche rep. said it was the latest "hot off the presses" version. I should be able to get the latest ver.
        Could be - they can probably get the latest version from Roche for you if they haven't already got it themselves.
        Originally posted by WaltL View Post
        So, have you done this type of assembly with the new version?
        No, not personally.

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