sff into fasta/qual files

[isalarubi]

Hi everybody!

I have a problem: when I have seen my fasta/qual files of my 454Jn run, I've realized that these files are 0kb, totally empty!!

anybody knows what happens?

thank you!!

Isabel

[glovesfox]

Did you solve this problem? How did you generate your .fasta and .qual files?

[isalarubi]

Hi Glovesfox!

Finally, I couldn't generate the files, so if you have the same problem, I can't say you anything.

Sorry!

Isabel

[sklages]

You still haven't provided any information on what you did. Has the run successfully finished? What have you done to create which fasta/qual files? What about your SFF files? Are these empty or not?

I don't have a crystal ball ;-)

[glovesfox]

I don't have the same problem - I am trying to help you!
You must have done something to generate fasta and qual files from the sff file. I'm guessing you used a tool called sfffile on the command line?

[ajthomas]

Some questions to help us understand so we can help you:

Are you talking about the .fna and .qual files that are automatically created as part of the process, or are these files you created later from the .sff file?

Have you looked at the run in the Run Browser? How did that look?

What is the file size of the .sff file?

[isalarubi]

Sorry!

The sff file only has 2kb. This run was a totally disaster, so I think that we can forget it.

Thanks so much, and sorry, I'm totally new at this and I don't understand the most of the thinks.