Paper: Fully automated process for barcoded libraries for 454

[RCJK]

Check this out for automated 454 general library prep:

http://genomebiology.com/2010/11/2/R15

"A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454"

[nickloman]

Very cool. I wish we could just buy it off the shelf and have it working tomorrow!

[dottomarco]

This is based on the old library protocol right? Now the new rapid library protocol solved a lot of issues.. but it wold be interesting to apply the 96 parallelization idea of the paper to the new rapid protocol

[james hadfield]

Fluidigm have a 454 amplicon prep method that allows you to make 48 libraries of up to 480 amplicons, barcoded and ready to sequence. All from one PCR.

[nickloman]

Oh yes, this thing? http://www.fluidigm.com/pr/2010/2010...essRelease.htm

Looks really handy for some of our apps.