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lara

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Join Date: Feb 2010

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coverage for amplicon seq?

03-08-2010, 06:23 AM

Hi SEQcommunity,

we're starting a 454 project to look for mutations/SNPs in several amplicons of a particular gene. We're not sure about the coverage needed. Roche told us we need a covergae of 1000 to detect a variant with 5% frequency. Does anyone got experience with this issue?

Thanks in advance
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