While prepping enriched beads for a GS Jr run last week, I messed up the addition of the polymerase. That's what I get for trying to hurry. Anyway, to fix my mistake I just opened a second sequencing kit and used the polymerase from it. Never prep a run without back-up reagents in the freezer, right?! Well, now I have a full sequencing kit without any polymerase... Grrrr...
Any suggestion on which polymerase, at what concentration, to use as a substitute for the one that comes in the kit? I called Roche Tech Support and DNA polI at normal concentration was suggested as a substitute, but they couldn't tell me exactly what is in their kit. Just hoping someone has experience with this and could tell me exactly what will work.
Any suggestion on which polymerase, at what concentration, to use as a substitute for the one that comes in the kit? I called Roche Tech Support and DNA polI at normal concentration was suggested as a substitute, but they couldn't tell me exactly what is in their kit. Just hoping someone has experience with this and could tell me exactly what will work.
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