Rapid library prep and Nimblegen enrichment

[Tom Haltern]

Can somebody help me in getting the new RL protocol compatible with the Nimblegen capture downstream? I tried to design the old Titanium adaptors myself with a T overhang but they didn't work obviously. Got no PCR product. Has somebody ever tried that approach successfully? Perhaps I made a mistake during oligo/adaptor design??? Thanks so much. Tom

[Zaag]

I think the primers you would normally use for you LM-PCR work on the rapid library as well. The biggest problem I see is that your blockers (Hybridization Enhancing Oligo's) won't work as well as they do for general libraries so if this would effect your ontarget you'll have to design them. Perhaps even per MID.