Can somebody help me in getting the new RL protocol compatible with the Nimblegen capture downstream? I tried to design the old Titanium adaptors myself with a T overhang but they didn't work obviously. Got no PCR product. Has somebody ever tried that approach successfully? Perhaps I made a mistake during oligo/adaptor design??? Thanks so much. Tom
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I think the primers you would normally use for you LM-PCR work on the rapid library as well. The biggest problem I see is that your blockers (Hybridization Enhancing Oligo's) won't work as well as they do for general libraries so if this would effect your ontarget you'll have to design them. Perhaps even per MID.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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