Can somebody help me in getting the new RL protocol compatible with the Nimblegen capture downstream? I tried to design the old Titanium adaptors myself with a T overhang but they didn't work obviously. Got no PCR product. Has somebody ever tried that approach successfully? Perhaps I made a mistake during oligo/adaptor design??? Thanks so much. Tom
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I think the primers you would normally use for you LM-PCR work on the rapid library as well. The biggest problem I see is that your blockers (Hybridization Enhancing Oligo's) won't work as well as they do for general libraries so if this would effect your ontarget you'll have to design them. Perhaps even per MID.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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