Hi everyone,
I'm new to sequencing and am wondering if I have estimated my genome size and therefore expected coverage for 454 sequencing correctly.
From flow cytometry I know that my plant is 25pg/2c=12.5pg/c
if 1pg=978Mb
then 978*12.5=12225Mb.
This is what I believe my genome size to be.
Therefore if I did 1/8th of a plate with an estimated throughput of 30-55Mb, my expected coverage is then:
30/12225-55/12225 = 0.00245x-0.0045X coverage.
This seems very small and I am wondering if I did this all wrong??
Thanks in advance for your help!
I'm new to sequencing and am wondering if I have estimated my genome size and therefore expected coverage for 454 sequencing correctly.
From flow cytometry I know that my plant is 25pg/2c=12.5pg/c
if 1pg=978Mb
then 978*12.5=12225Mb.
This is what I believe my genome size to be.
Therefore if I did 1/8th of a plate with an estimated throughput of 30-55Mb, my expected coverage is then:
30/12225-55/12225 = 0.00245x-0.0045X coverage.
This seems very small and I am wondering if I did this all wrong??
Thanks in advance for your help!
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