I am planning to acquire draft genome sequence of a bacterial strain by using GS FLX titanium sequencer. My question is whether initially sheared genomic dna can be safely used for genome sequencing and assembly by 454 technology. By 'initially', I mean genomic DNA was sheared during extraction process. I attach gel image of my genomic dna samples (marker is lambda dna digested with EcoRI and HindIII).
Although I know that dna fragmentation step is involved in standard genome sequencing by GS FLX titanium, I am concerned because I think it might be possible that shearing during extraction can affect randomness of standard fragmentation step and so on.
Thanks in advance.
Although I know that dna fragmentation step is involved in standard genome sequencing by GS FLX titanium, I am concerned because I think it might be possible that shearing during extraction can affect randomness of standard fragmentation step and so on.
Thanks in advance.
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