11 results in 0.0022 seconds.
-
NEB Fragmentase mitogenome advice
Hi, I am currently working with mitogenome sequencing of small mammals. I have amplified 5 PCR amplicons of the mitogenome around 4-5kb fragment lengths....Last edited by frascom; 06-07-2019, 07:26 AM. -
Shearing digested DNA for RADseq
Hello,
I am making libraries for RADseq. I am trying out the Covaris ME220 for shearing the digested DNA. The shearing seems to be highly... -
Covaris tubes
Has anyone tried an alternative tube type or plate other than the glass microTUBEs for shearing gDNA using the Covaris LE220 series?
... -
genomic DNA shearing with Covaris 12*12 tubes
Hello, I am calibrating a new protocol and I need to shear my cross-linked DNA (2*10^7 RAW cell line) to an average size of 1000 bps. I'm using Covaris... -
Covaris M220 or S220?
Hi all!
Working in a lab about to install the HiSeq system.
We expect a throughput of about 48 samples a week.
... -
Differences between sonicators?
Hi
I'm new here and looking for advice on fragmenting chromatin. We plan to do quite a bit of ChIPseq to identify transcription factor... -
covaris shearing range
I was wondering if anyone has used the covaris for a shearing range of 2-5kb for DNA and what were your results. I need this size range to start a protocol... -
covaris shearing settings for paired end
Hello,
Does anyone use the Covaris to shear their samples in the Roche 3kb paired end library preparation step of circularised DNA fragmentation?... -
Can I use sheared genomic DNA as starting material of bacterial genome sequencing?
I am planning to acquire draft genome sequence of a bacterial strain by using GS FLX titanium sequencer. My question is whether initially sheared genomic... -
LCL vs whole blood samples? (shearing differences)
Dear all,
I sheared lymphoblastoid cell line (LCL) samples and whole blood samples by covaris S2. Then, I ran bioanalyzer to check their length.... -
What is at the ends of sonicated DNA?
Given the poor molar yields of typical next gen library construction methods, I have often wondered whether end-repair after shearing was an issue. There...
