Tweet
There is definitely a variability between the SV and LV kits. With the new LV oil, I see a distinct clear layer after the emulsion PCR, but with SV, there is not a clear layer in all of them. Not sure if the cpb are transferable from SV to LV. Only thing I suggest is to aim low when it comes to cpb and CAREFULLY detect the broken emulsions (I explained how I do this above) and make sure to take them out before breaking.
-
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
Comment