There is definitely a variability between the SV and LV kits. With the new LV oil, I see a distinct clear layer after the emulsion PCR, but with SV, there is not a clear layer in all of them. Not sure if the cpb are transferable from SV to LV. Only thing I suggest is to aim low when it comes to cpb and CAREFULLY detect the broken emulsions (I explained how I do this above) and make sure to take them out before breaking.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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