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There is definitely a variability between the SV and LV kits. With the new LV oil, I see a distinct clear layer after the emulsion PCR, but with SV, there is not a clear layer in all of them. Not sure if the cpb are transferable from SV to LV. Only thing I suggest is to aim low when it comes to cpb and CAREFULLY detect the broken emulsions (I explained how I do this above) and make sure to take them out before breaking.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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