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Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)
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Started by ECO, 11-05-2007, 11:40 PM
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by chelseaclark
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Started by ECO, 12-29-2008, 08:55 PM
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by ParkerKB
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Started by ECO, 09-27-2008, 05:28 PM
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01-05-2011, 09:34 AM
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01-03-2011, 05:37 PM
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12-21-2010, 10:14 AM
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Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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