If it is correct, I'd like to know how the p5 and p7 tethered stands were cut?
Immobilised oligos on paired end Flowcell surface:
Oligo ‘C’: 5’-PS-TTTTTTTTTTAATGATACGGCGACCACCGAGAUCTACAC-3’
(U = 2-deoxyuridine), Linearization of oligo C with “USER” enzyme retains strand 1, attached to “D” oligo.
Oligo ‘D’: 5’-PS-TTTTTTTTTTCAAGCAGAAGACGGCATACGAGoxoAT-3’,
(Goxo = 8-oxoguanine) Second strand is cleaved at the 8-oxoguanine in oligo 'D' using Fpg enzyme.
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