Can I multiplex bacterial genomes on MiSeq? If so, how many ? Thank you!
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Uh...how much coverage will be useful for you?
Today = 8-9M PF read pairs (2x150bp from each) = ~2.6GB = ~566x coverage of one E.coli sized genome (4.5MB). Divide at will. -
What does PF stand for? Sorry, I'm a newbie in NGS.Comment
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It means Pass Filter - not all reads generated pass the filtering process eg a run could generate 10 million reads but only 9 million are passed by the quality filtering process.Originally posted by eosin View PostComment
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