I'm wondering if anyone has data on the approximate yield for a single cluster during bridge amplification (i.e. ng/cluster for a theoretical 100bp target). In the context of liquid PCR I imagine it's limited due to physical contraints vs. diffusion but if anyone has data on this, I'd be really interested. thanks!
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This Illumina document (PDF) says "up to 1000 identical molecules" of each DNA per cluster, so sub-femtogram.
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by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
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10-14-2026, 09:55 AM -
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