I'm wondering if anyone has data on the approximate yield for a single cluster during bridge amplification (i.e. ng/cluster for a theoretical 100bp target). In the context of liquid PCR I imagine it's limited due to physical contraints vs. diffusion but if anyone has data on this, I'd be really interested. thanks!
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This Illumina document (PDF) says "up to 1000 identical molecules" of each DNA per cluster, so sub-femtogram. -
Thanks!
Thanks for the prompt reply! Exactly the info I needed.Comment
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