Long insert library sequencing on Miseq (~1 KB)

[rnaeye]

Hi all,
I have two libraries, one 800 bp and other one is 1 KB including adapter length. I am hoping to sequence them on Miseq instrument (V2). I was wondering if anyone has any recommendation on what concentration of library I should load on flow cell. Thank you.

[ScottC]

Perhaps this will help:

http://res.illumina.com/documents/pr...validation.pdf

[james hadfield]

The clustering favours small molecules so if you ahvea distribution of fragment sizes you may well see an imbalance compared to a Bioanalyser trace for instance. I'd go for a slightly lower cluster density than normal and aim for abou 800/mm. The 1Kb is pushing the limits of what will cluster!