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  • WhiteSeal
    Member
    • Jul 2013
    • 14

    #1

    What is a good run? Do you have a QC to determine that.

    Alright, I was in a different thread and at one point the discussion went slightly of topic but it was clear to me that everyone had a different opinion on what is a good MiSeq/HiSeq run. So in a Good Mythical Morning sense "let's talk bout that".

    In your own opinion what makes a good run? Also do you have a specific QC to determine that.
  • Baseless
    Member
    • Feb 2010
    • 31

    #2
    A ok run is when its within manufacturer specs both output and base quality wise. A good run is slightly better. And an excellent run is when no one complains about data afterwards.
    Our best so far was ~360GB off a HiSeq cell 2x101+index.

    Comment

    • Zaag
      Senior Member
      • Nov 2009
      • 112

      #3
      High percentage Q30 or higher bases
      High amount of data
      High percentage reads that reach full length

      Comment

      • niroshan
        Junior Member
        • Jun 2011
        • 9

        #4
        This may help you. Go to this page:
        User courses, WebExes, and videos for Illumina products and services


        and click on "MiSeq: Does My Run Look Good?"

        Comment

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