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What is a good run? Do you have a QC to determine that.

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SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
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  • What is a good run? Do you have a QC to determine that.

    Alright, I was in a different thread and at one point the discussion went slightly of topic but it was clear to me that everyone had a different opinion on what is a good MiSeq/HiSeq run. So in a Good Mythical Morning sense "let's talk bout that".

    In your own opinion what makes a good run? Also do you have a specific QC to determine that.

  • #2
    A ok run is when its within manufacturer specs both output and base quality wise. A good run is slightly better. And an excellent run is when no one complains about data afterwards.
    Our best so far was ~360GB off a HiSeq cell 2x101+index.

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    • #3
      High percentage Q30 or higher bases
      High amount of data
      High percentage reads that reach full length

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      • #4
        This may help you. Go to this page:
        User courses, WebExes, and videos for Illumina products and services


        and click on "MiSeq: Does My Run Look Good?"

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