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  • What is a good run? Do you have a QC to determine that.

    Alright, I was in a different thread and at one point the discussion went slightly of topic but it was clear to me that everyone had a different opinion on what is a good MiSeq/HiSeq run. So in a Good Mythical Morning sense "let's talk bout that".

    In your own opinion what makes a good run? Also do you have a specific QC to determine that.

  • #2
    A ok run is when its within manufacturer specs both output and base quality wise. A good run is slightly better. And an excellent run is when no one complains about data afterwards.
    Our best so far was ~360GB off a HiSeq cell 2x101+index.

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    • #3
      High percentage Q30 or higher bases
      High amount of data
      High percentage reads that reach full length

      Comment


      • #4
        This may help you. Go to this page:
        User courses, WebExes, and videos for Illumina products and services


        and click on "MiSeq: Does My Run Look Good?"

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