Hi guys,
I'm planning a bacterial 16s rRNA amplicon sequencing project using Nextseq 500 with 300-cycles reagents. Now I'm trying to figure out how to choose an amplicon size and what the best run type is (single or paired end? If the latter, should the two paired end reads partially overlap?). Are there any basic "rules" or reliable primer sets available? Any suggestions/references would be appreciated.
I'm planning a bacterial 16s rRNA amplicon sequencing project using Nextseq 500 with 300-cycles reagents. Now I'm trying to figure out how to choose an amplicon size and what the best run type is (single or paired end? If the latter, should the two paired end reads partially overlap?). Are there any basic "rules" or reliable primer sets available? Any suggestions/references would be appreciated.
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