Hello everyone,
I am a new member of SEQanswers. I am planning to run MiniSeq system for the first time. Unluckily, I have a big problem. My pooled library is a combination of 2 kinds of library, which were prepared by 2 library preps kits. The first one is Nextera XT and the second one is NEB Ultra II. The problem is that they have different lengths in index sequences. Nextera XT indexes contain 8 nucleotides, meanwhile NEB Ultra II was designed with only 6 nucleotide. How can I run those libraries at the same time, on the same flow cell. Please help me!!!
I am a new member of SEQanswers. I am planning to run MiniSeq system for the first time. Unluckily, I have a big problem. My pooled library is a combination of 2 kinds of library, which were prepared by 2 library preps kits. The first one is Nextera XT and the second one is NEB Ultra II. The problem is that they have different lengths in index sequences. Nextera XT indexes contain 8 nucleotides, meanwhile NEB Ultra II was designed with only 6 nucleotide. How can I run those libraries at the same time, on the same flow cell. Please help me!!!
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