Custom primers for NextSeq: will they work on MiSeq and HiSeq?
I am wondering whether my experiment (which was originally designed to work on a NextSeq) will work on MiSeq and HiSeq.
It is a library prepared with MIP technique and the PCR primers are:
CAAGCAGAAGACGGCATACGAGATNNNNNNNNACACGCACGATCCGACGGTAGTGT
AATGATACGGCGACCACCGAGATCTACACATACGAGATCCGTAATCGGGAAGCTGAAG
And the question is whether they actually will bind to all MiSeq, HiSeq and NextSeq flow cells? I've heard somewhere that these sequences are different.
Next stuff, sequencing primers. These are custom oligos with sequences below:
FOR* CATACGAGATCCGTAATCGGGAAGCTGAAG*
REV* ACACGCACGATCCGACGGTAGTGT*
INDEX*PRIMER ACACTACCGTCGGATCGTGCGTGT*
Will they work? I would be grateful for a quick answer.
Thanks!
Anna
It is a library prepared with MIP technique and the PCR primers are:
CAAGCAGAAGACGGCATACGAGATNNNNNNNNACACGCACGATCCGACGGTAGTGT
AATGATACGGCGACCACCGAGATCTACACATACGAGATCCGTAATCGGGAAGCTGAAG
And the question is whether they actually will bind to all MiSeq, HiSeq and NextSeq flow cells? I've heard somewhere that these sequences are different.
Next stuff, sequencing primers. These are custom oligos with sequences below:
FOR* CATACGAGATCCGTAATCGGGAAGCTGAAG*
REV* ACACGCACGATCCGACGGTAGTGT*
INDEX*PRIMER ACACTACCGTCGGATCGTGCGTGT*
Will they work? I would be grateful for a quick answer.
Thanks!
Anna