Hi,
I am using the NexteraXT kit to prep mtDNA. I just did a test prep of some samples and had them run on a HS DNA Chip on the Bioanalyzer (see attachment).
I would like a second opinion on these. Does the libraries look undertagmentated?
(see past sample B, I screwed that one up...)
A: Following the procedure recommendations (1ng)
C: Half of the recommendations (0.5ng)
D: Quarter of the recommendations (0.25ng)
Also, quantifying the libraries by Qubit revealed them to be on the low end, just under 2nM with standard 12cycles PCR. Should I be concerned that the output of the prep is so low?
Thanks!
I am using the NexteraXT kit to prep mtDNA. I just did a test prep of some samples and had them run on a HS DNA Chip on the Bioanalyzer (see attachment).
I would like a second opinion on these. Does the libraries look undertagmentated?
(see past sample B, I screwed that one up...)
A: Following the procedure recommendations (1ng)
C: Half of the recommendations (0.5ng)
D: Quarter of the recommendations (0.25ng)
Also, quantifying the libraries by Qubit revealed them to be on the low end, just under 2nM with standard 12cycles PCR. Should I be concerned that the output of the prep is so low?
Thanks!
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