Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • ikripp
    Member
    • Jan 2018
    • 19

    #1

    MiSeq Error in LanePump

    Hi All,

    We just ran a MiSeq 2x300bp amplicon sequencing run over the weekend and it has completed with decent specs but has shown an error that says the following:
    "Error in LanePump. Syringe pump value failed to move. Valve overload. Command = '/1Qe\r', reply = 'j'.

    I've seen most other MiSeq errors before, and have already sent an email to illumina tech support. I was wondering if anyone else had experienced this before -if you have any insight into how it may have impacted the output etc.

    Thanks!
  • ikripp
    Member
    • Jan 2018
    • 19

    #2
    Update:
    Tech support have identified it as a software or hardware error and told me to power cycle.
    Unfortunately its a hardware error so now I have an engineer coming to fix the machine.

    Just in case it happens to anyone else!

    Comment

    • SwineFlu
      Junior Member
      • Nov 2017
      • 1

      #3
      Thanks ikripp,
      I got the exact problem few days ago and already called tech support. Just wonder to know how did they fix it - replacing the pump?

      Comment

      • ikripp
        Member
        • Jan 2018
        • 19

        #4
        Originally posted by SwineFlu View Post
        Thanks ikripp,
        I got the exact problem few days ago and already called tech support. Just wonder to know how did they fix it - replacing the pump?
        They ended up replacing the hard drive and that resolved the issue. I believe the pump error was a result of the hard drive failing. Tech support instructed me to power cycle the machine and when I did it wouldn't turn back on! Then they came and replaced the hard drive and we had to reconfigure the machine again.
        On a positive note it is now running smoothly!
        Last edited by ikripp; 08-16-2018, 10:59 PM.

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2




          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
          Yesterday, 06:46 AM
        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        29 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-28-2026, 09:51 AM
        0 responses
        20 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 11:13 AM
        0 responses
        71 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 10:48 AM
        0 responses
        48 views
        0 reactions
        Last Post SEQadmin2  
        Working...