Hi,
I'm very new to sequencing so apologies if my question is naive.
I am trying to sequence whole human genome samples just to test the reproducibility of my library preparation method. I'm not interested in mutation frequency.
1. Would single-index adapters be good enough for pooling 6-8 samples, or do I need dual index?
2. What depth of coverage would I need to just see if my DNA is good enough to sequence. Could I use MiSeq or would I need HiSeq?
Thanks in advance for any help!
I'm very new to sequencing so apologies if my question is naive.
I am trying to sequence whole human genome samples just to test the reproducibility of my library preparation method. I'm not interested in mutation frequency.
1. Would single-index adapters be good enough for pooling 6-8 samples, or do I need dual index?
2. What depth of coverage would I need to just see if my DNA is good enough to sequence. Could I use MiSeq or would I need HiSeq?
Thanks in advance for any help!