Dear all,
I was wondering if there was any tool to help me reduce the PhiX% used in our NextSeq 500 runs? We run similar samples continuously, with "low" diversity as we enrich specific regions by hybrid capture. Instead of empirical & successive testings to reduce the PhiX%, can I guestimate the "ideal" PhiX% to add before reaching the slippery slope?
Thank you for your help.
Gaetan
I was wondering if there was any tool to help me reduce the PhiX% used in our NextSeq 500 runs? We run similar samples continuously, with "low" diversity as we enrich specific regions by hybrid capture. Instead of empirical & successive testings to reduce the PhiX%, can I guestimate the "ideal" PhiX% to add before reaching the slippery slope?
Thank you for your help.
Gaetan
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