What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?
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The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.Leave a comment:
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maybe you should start specifying which technology you're using on which instrument...Leave a comment:
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Adapters sequence
Hi all!
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
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