Hi all!
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
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maybe you should start specifying which technology you're using on which instrument... -
Ion Torrent, Amplicon sequencing))Comment
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Assuming it's Ion Torrent as it's in the Ion Torrent thread...Comment
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In this manual are only the primer sequence, ... but adaptor sequence absence? is they full complementary and same size?Last edited by Chartiza; 02-01-2012, 03:53 AM.Comment
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The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.Comment
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having the sequence of the (Y) adaptors would be handy!Comment
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What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?
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