Hi all!
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
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maybe you should start specifying which technology you're using on which instrument... -
Ion Torrent, Amplicon sequencing))Comment
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Assuming it's Ion Torrent as it's in the Ion Torrent thread...Comment
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In this manual are only the primer sequence, ... but adaptor sequence absence? is they full complementary and same size?Last edited by Chartiza; 02-01-2012, 03:53 AM.Comment
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The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.Comment
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having the sequence of the (Y) adaptors would be handy!Comment
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What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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