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Hi all!
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
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maybe you should start specifying which technology you're using on which instrument... -
Ion Torrent, Amplicon sequencing))Comment
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Assuming it's Ion Torrent as it's in the Ion Torrent thread...Comment
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In this manual are only the primer sequence, ... but adaptor sequence absence? is they full complementary and same size?Last edited by Chartiza; 02-01-2012, 03:53 AM.Comment
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The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.Comment
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having the sequence of the (Y) adaptors would be handy!Comment
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What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?
http://seqanswers.com/forums/showthread.php?t=16450Comment
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
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