Cancer panel v2,low quality,316v2
Hi, these days ,I run some 316v2 chips on PGM, and the %low quality show very high, ranged from 27-40%! Can anyone tell me why?
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We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard
JPC -
Thank you for your reply!Originally posted by JPC View Post
We had try that before, but it didn't work!
by the way, how do you quantify the library, 2100 or qubit?Comment
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qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)
JPCComment
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You probably need to check amplifiability of your DNA. Using the RNaseP kit will help out with that. Crap in, crap out.Comment
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