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Priming Flongle Flowcell

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  • Priming Flongle Flowcell

    Hi!
    I hope you are very well, what protocols are there for priming the Flongle Flowcells?
    Thanks.

  • #2
    Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.

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    • #3
      Originally posted by gringer View Post
      Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.
      it was very useful, thank you very much

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