Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Priming Flongle Flowcell

    Hi!
    I hope you are very well, what protocols are there for priming the Flongle Flowcells?
    Thanks.

  • #2
    Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.

    Comment


    • #3
      Originally posted by gringer View Post
      Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.
      it was very useful, thank you very much

      Comment


      • #4
        Hello!
        Regarding your question, Flongle flow cells are compatible with both R9.4 and R10.3 chemistry, and the protocol for priming them depends on the specific chemistry you are using.
        For R9.4 chemistry, the Flongle flow cells can be primed using the protocol for priming MinION flow cells, which involves flushing the flow cell with running buffer and then washing with loading buffer. The detailed protocol can be found in the MinION flow cell user guide, available on the Oxford Nanopore Technologies website.
        For R10.3 chemistry, the priming protocol is slightly different and involves priming the flow cell with EP buffer and then washing with loading buffer. The detailed protocol for priming R10.3 Flongle flow cells can also be found on the Oxford Nanopore Technologies website.
        It is important to carefully follow the priming protocols for your specific chemistry to ensure optimal performance and to prevent damage to the flow cell.backrooms game

        Comment


        • #5
          As far as I'm aware, Flongle flow cells have never been available for R9.4 nor R10.3. R9.4 was an earlier pore technology that stopped being used by ONT around 2019. R10.3 I'm less sure about. It might have been an earlier R10 technology, but was never offered on Flongle (except possibly to a sprinkling of labs for early-access testing).

          Flongle flow cells are currently available on the ONT store in R9.4.1 and R10.4.1 pore formats ($810 USD for 12 flow cells).

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM
          • seqadmin
            Techniques and Challenges in Conservation Genomics
            by seqadmin



            The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

            Avian Conservation
            Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
            03-08-2024, 10:41 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Yesterday, 06:37 PM
          0 responses
          10 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, Yesterday, 06:07 PM
          0 responses
          9 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-22-2024, 10:03 AM
          0 responses
          50 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-21-2024, 07:32 AM
          0 responses
          67 views
          0 likes
          Last Post seqadmin  
          Working...
          X