Priming Flongle Flowcell

[d_araneda_r]

Hi!
I hope you are very well, what protocols are there for priming the Flongle Flowcells?
Thanks.

[gringer]

Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.

[d_araneda_r]

Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.

it was very useful, thank you very much