Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • lguzmanz
    Junior Member
    • Mar 2016
    • 4

    #1

    Canu error: 'failed to create the overlap store'

    Trying to run the following command:

    $ canu -trim -p PacBio10_trimmed -d PacBio10_trimmed genomeSize=200000 -pacbio-corrected /work/03868/lguzmanz/liz_trials/PacBio_correctedreads/corrected_Canu_pacbio10.correctedReads.fasta.gz "useGrid=false"

    Got the following error message:


    ERROR:
    ERROR: Failed with exit code 1.

    ERROR:

    Don't panic, but a mostly harmless error occurred and canu failed.

    Disk space available: 17025761.9 GB

    Last 50 lines of the relevant log file (/work/03868/lguzmanz/liz_trials/april_20/PacBio10_trimmed/trimming/PacBio10_trimmed.ovlStore.err):

    Summing overlap counts for 1 reads from '/work/03868/lguzmanz/liz_trials/april_20/PacBio10_trimmed/trimming/1-overlapper/001/000001.counts'.
    Found no overlaps to sort.

    canu failed with 'failed to create the overlap store'.


    I had to manually correct 18 PacBio reads because the correct/trim/assemble default canu code ran into issues, now that I look back on it, it was probably the same error with the "failed to create overlap store" when trimming.

    Any help would be greatly appreciated!

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    Today, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
71 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...