Tweet
We are a core facility that receives samples from multiple labs. We have one lab in particular that submits which are ran on a 3730XL. We are trying to determine what is happening with these particular results. The first time that we run their plate the results don't look particularly good but if we put that same plate back into the ABI we see a much better signal. I've spoken with another facility who thought it was due to some contamination. I don't really know where this would be coming from as we run the bigdye reaction and cleanseq on all the plates and it's only seen with this particular lab's plates. I would really like to try to help troubleshoot this issue for them. I have attached images of the first and second run. Subsequent plates look pretty good on the first run but could also be improved on the second run. These are also being ran back to back.
-
The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i
...01-24-2023, 01:19 PM -
Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.
The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...01-09-2023, 03:10 PM