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I think i used to use 4.2.2. But i don't have the archive to install it (i didn't install it on our cluster).
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Hi,
I just want to reiterate how crazy double encoding is! Thought we were having problems with our aligner as the 'reads' weren't mapping to the reference. Why on earth did ABI pick those 4 letters? Why even double encode in the first place?!
Thanks Rick!Comment
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i hope people realize converting to fastq is probably one of the worst ways to analyze cs data.Comment
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SeqAA, could you describe your workflow?Comment
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SeqAA - agree.
People, if you want to analyse Solid data properly use colour space. If you're forced into the dark arts of base space conversion i.e. for de novo assembly I would strongly recommend reading the supplements of this paper:
Iverson et al. 2012, Science : Untangling genomes from metagenomes ....Comment
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I generally use galaxy to do most of my conversionsComment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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