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  • idonaldson
    Member
    • Oct 2009
    • 37

    #31
    I think i used to use 4.2.2. But i don't have the archive to install it (i didn't install it on our cluster).

    Comment

    • flashton
      Member
      • Feb 2011
      • 10

      #32
      Hi,

      I just want to reiterate how crazy double encoding is! Thought we were having problems with our aligner as the 'reads' weren't mapping to the reference. Why on earth did ABI pick those 4 letters? Why even double encode in the first place?!

      Thanks Rick!

      Comment

      • SeqAA

        #33
        i hope people realize converting to fastq is probably one of the worst ways to analyze cs data.

        Comment

        • flashton
          Member
          • Feb 2011
          • 10

          #34
          SeqAA, could you describe your workflow?

          Comment

          • colindaven
            Senior Member
            • Oct 2008
            • 417

            #35
            SeqAA - agree.

            People, if you want to analyse Solid data properly use colour space. If you're forced into the dark arts of base space conversion i.e. for de novo assembly I would strongly recommend reading the supplements of this paper:

            Iverson et al. 2012, Science : Untangling genomes from metagenomes ....

            Comment

            • vswilliamson
              Junior Member
              • Jun 2010
              • 2

              #36
              I generally use galaxy to do most of my conversions

              Comment

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