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I think i used to use 4.2.2. But i don't have the archive to install it (i didn't install it on our cluster).
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Hi,
I just want to reiterate how crazy double encoding is! Thought we were having problems with our aligner as the 'reads' weren't mapping to the reference. Why on earth did ABI pick those 4 letters? Why even double encode in the first place?!
Thanks Rick!Comment
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i hope people realize converting to fastq is probably one of the worst ways to analyze cs data.Comment
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SeqAA, could you describe your workflow?Comment
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SeqAA - agree.
People, if you want to analyse Solid data properly use colour space. If you're forced into the dark arts of base space conversion i.e. for de novo assembly I would strongly recommend reading the supplements of this paper:
Iverson et al. 2012, Science : Untangling genomes from metagenomes ....Comment
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I generally use galaxy to do most of my conversionsComment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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