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No update sorry, we are awaiting a firmware upgrade before running the machine. I have ordered oligos to block the whole sequence, but this will become expensive when multiplexing. We can then try using N's across the barcode section or possibly to batches of barcodes where R, Y, K, M etc can be utilised (as recommended by a contact in Liverpool CGR). -
Ran into the same problem, we are testing blocking the whole thing, but haven't got results yet (capture done, seq next week). This post is a few weeks old, do you have an update?
ABI's TargetSeq uses no 3' end modification in their blockers, which also differs to the Baylor protocolLeave a comment:
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5500 EZ Exome Hybridisation Enhancers
When using the 5500 adapters and the Roche EZ Exome capture kit should I block the whole sequence of the P1 (41bp) and Standard (53bp) adapters during hybridisation? or can I just block the 3' end of each adapter...or just the Library PCR section?
I know Baylor used truncated adapters with their SOLiD 4 protocol and I could use this at the P1 end but they don't use the P2/barcode/IA.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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