-
No update sorry, we are awaiting a firmware upgrade before running the machine. I have ordered oligos to block the whole sequence, but this will become expensive when multiplexing. We can then try using N's across the barcode section or possibly to batches of barcodes where R, Y, K, M etc can be utilised (as recommended by a contact in Liverpool CGR). -
Ran into the same problem, we are testing blocking the whole thing, but haven't got results yet (capture done, seq next week). This post is a few weeks old, do you have an update?
ABI's TargetSeq uses no 3' end modification in their blockers, which also differs to the Baylor protocolLeave a comment:
-
5500 EZ Exome Hybridisation Enhancers
When using the 5500 adapters and the Roche EZ Exome capture kit should I block the whole sequence of the P1 (41bp) and Standard (53bp) adapters during hybridisation? or can I just block the 3' end of each adapter...or just the Library PCR section?
I know Baylor used truncated adapters with their SOLiD 4 protocol and I could use this at the P1 end but they don't use the P2/barcode/IA.
-
Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.
Methylation Detect...05-31-2023, 10:46 AM -
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...05-19-2023, 10:03 AM
Leave a comment: