Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • 5500 EZ Exome Hybridisation Enhancers

    When using the 5500 adapters and the Roche EZ Exome capture kit should I block the whole sequence of the P1 (41bp) and Standard (53bp) adapters during hybridisation? or can I just block the 3' end of each adapter...or just the Library PCR section?

    I know Baylor used truncated adapters with their SOLiD 4 protocol and I could use this at the P1 end but they don't use the P2/barcode/IA.

  • #2
    Ran into the same problem, we are testing blocking the whole thing, but haven't got results yet (capture done, seq next week). This post is a few weeks old, do you have an update?
    ABI's TargetSeq uses no 3' end modification in their blockers, which also differs to the Baylor protocol

    Comment


    • #3
      No update sorry, we are awaiting a firmware upgrade before running the machine. I have ordered oligos to block the whole sequence, but this will become expensive when multiplexing. We can then try using N's across the barcode section or possibly to batches of barcodes where R, Y, K, M etc can be utilised (as recommended by a contact in Liverpool CGR).

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Latest Developments in Precision Medicine
        by seqadmin



        Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

        Somatic Genomics
        “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
        05-24-2024, 01:16 PM
      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        05-06-2024, 07:48 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 05-24-2024, 07:15 AM
      0 responses
      15 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-23-2024, 10:28 AM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-23-2024, 07:35 AM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-22-2024, 02:06 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Working...
      X