Hello All, just in case anyone in interested.....we are able to get 10 exomes at 50X coverage from a single flowcell (with the standard 1um microbeads).
We aren't getting there every time, we count beads on the nanodrop, we think we've got 400M per lane but the lanes are often quite pale after loading.....we haven't figured out what's going wrong here yet?
When it works well, one lane gives us 180 - 200M reads, 18% don't map (using LifeScope and ECC).
Is anyone doing considerably better?....would you recommend the nanobeads to improve our figures?
Is anyone doing worse? I'm happy to share what I have learned!
JPC
We aren't getting there every time, we count beads on the nanodrop, we think we've got 400M per lane but the lanes are often quite pale after loading.....we haven't figured out what's going wrong here yet?
When it works well, one lane gives us 180 - 200M reads, 18% don't map (using LifeScope and ECC).
Is anyone doing considerably better?....would you recommend the nanobeads to improve our figures?
Is anyone doing worse? I'm happy to share what I have learned!
JPC
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