miRNA Sequence Read Number?

[mmb_psu]

Hello,

I am currently setting up my first SOLiD sequencing experiment, and wondered if anyone had any information regarding the recommended number of reads per sequence.
I am looking at ~20bp bovine microRNA and am likely to find between 250-400 unique sequences out of the RNA that I am sequencing, with a total of 10 separate samples to be sequenced at once. My options are one quarter of a plate, which I am told is equivalent to about 25 million reads, or a half of a plate for 50 million reads.
I can't seem to find any papers that outline this process, so was wondering if anyone knew how many reads per sequence is considered necessary to have good quality data?
Thanks!

[JDS]

In general protocol of SOLiD v3.0, the beads density should be 150K/panel.

So you can calculate the beads number ( tags number after sequencing ) depend on which kind of slide you run.

Full slide: 2357 panels = 354M tags (FG, *2 for MP )
1/4 slide : 459 panels / spot = 69M tags (FG, *2 for MP )

notics that the calculation is according to the beads density and the running quality.

Hope it can help you.

[mmb_psu]

Thank you! That was the missing piece that I needed.

[DNADEB]

I believe the recommended amount is 130 to 135K per panel. Be aware as well that only 50 to 60% are mappable.