SOLiD5500 (total) meltdown

[Zaag]

Hello,

we are experiencing problems with our 2 SOLiD5500 machines since end of july. It is getting extremely frustrating because one run the reverse quality is crap (<40% good best), another one the forward is crap (<20% good best)and the next one the barcode is crap (<60% good best).

Is there anyone else experiencing problems with their machines when comparing results to previous ones?

Sidenote: we do see that lane 1 is routinely better than lane 6, although lifetech assured us this was impossible.

[snetmcom]

Are you certain the library titration was correct? Best beads % can be low from too much DNA in bead prep.

[Zaag]

Yes I am sure the samples are good, we did run them previously without problems. The noise/signal and on axis are all ok.

The problem does not appear to be the machine so now we are testing another EZbead station and another lot-nr chemistry.

[Zaag]

I give up.

[snetmcom]

that does not look like solid data. shouldn't there be cycles of quality?

[Zaag]

You mean like this?



Last bar of the first ligation cycle is base 71
Last bar of the second ligation cycle is base 72

etc.