Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • saha
    Junior Member
    • Jan 2010
    • 5

    #1

    Mapping to SOLiD reads to draft genome

    I am presently involved in hybrid assembly and want to map my SOLiD reads against ROCHE contigs (length more than 1000bp). I approach is to map the reads with corona_lite, but facing problem with the cmap file. I found a small paragraph regarding this particular situation in corona_lite documentation but it is not that illustrative. Usually for mapping against human genome we take cmap as follows:

    1 1 path to reference for each chr. path to the double encoded file

    But for a draft genome i am confused. it tried with this following approach but i failed. i present cmap that i tried was:

    1 contig0001 path to the multifasta contig file

    Any help and guidance will be appreciable.

    with regards,
    saha
  • westerman
    Rick Westerman
    • Jun 2008
    • 1104

    #2
    Either make a very large cmap file with one line per contig file. Or put all of the contigs into one file and separate them by 'N's; that is the file will look like one large sequence. I usually do the latter. If you are clever with how many N's you use to separate each contig then you can get information from the mapping file; e.g., map to bases 1-1323 means contig#1, bases 1500-2300 contig #2, etc.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into
      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 08-06-2026, 07:41 AM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-03-2026, 10:13 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-31-2026, 02:55 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Working...